Composite

Part:BBa_K3763024

Designed by: Guokai Wang   Group: iGEM21_WHU-China   (2021-10-18)


the expression operator in pET26b+ and CDS of PctA, with OmpA signal peptide

Based on BBa_3763023,we planed to optimize the ability of leader peptide,so we change the native sec-dependent leader peptide into OmpA leader peptide which is ofetn used to guide recombinant proteins secreted out of the engineered bacteria.One of the reason that we chose OmpA leader peptide is that our engineered bacteria are E.coli which have OmpA proteins, so the secretion might be facilitated more than the native sec-dependent leader peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 326
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 310


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Parameters
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