Composite

Part:BBa_K376032

Designed by: Anisha Katyal, Andrew Kirk, Erik McCann, Lauren Rossi, Becky Hennessey   Group: iGEM10_Penn_State   (2010-10-27)


Oxygen Sensing AFP Generator+LuxI+Tet Inverter+ Lambda Repressor+LuxR Generator

This construct constitutively expresses the lambda repressor unless there is a POPS input triggered by the presence of Oxygen. This POPS input will also start the expression of LuxI. LuxR will also be constitutively expressed by a medium constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3067
    Illegal NheI site found at 3090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None