Composite

Part:BBa_K3753017

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)


UASTEF-pTEF-GFP-CYC1

A reporter pathway in Saccharomyces cerevisiae used to characterize the activity of UASTEF-pTEF. For detailed characterization data, please visit UASTEF-pTEF BBa_K3753012.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 629
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167
    Illegal BsaI.rc site found at 378
    Illegal BsaI.rc site found at 587
    Illegal BsaI.rc site found at 2305


[edit]
Categories
Parameters
chassisSaccharomyces cerevisiae