Composite

Part:BBa_K3748077

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pRAD27-αMF-BA-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 1,38021E-05 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1167
    Illegal XbaI site found at 1649
    Illegal SpeI site found at 1806
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1167
    Illegal SpeI site found at 1806
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2430
    Illegal EcoRI site found at 1167
    Illegal BglII site found at 699
    Illegal BamHI site found at 1093
    Illegal XhoI site found at 948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1167
    Illegal XbaI site found at 1649
    Illegal SpeI site found at 1806
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1167
    Illegal XbaI site found at 1649
    Illegal SpeI site found at 1806
    Illegal AgeI site found at 1731
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None