Composite

Part:BBa_K3748069

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pRPL18B-αMFΔ-BS-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 0,000154906 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1494
    Illegal XbaI site found at 869
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1494
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2745
    Illegal EcoRI site found at 1494
    Illegal BglII site found at 705
    Illegal BglII site found at 1966
    Illegal BglII site found at 2136
    Illegal BamHI site found at 1986
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1494
    Illegal XbaI site found at 869
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1494
    Illegal XbaI site found at 869
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None