Composite

Part:BBa_K3748065

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pPGK1-αMF_no_EAEA-BA-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 1,39692E-05 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1161
    Illegal XbaI site found at 1643
    Illegal SpeI site found at 1800
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1161
    Illegal SpeI site found at 1800
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2424
    Illegal EcoRI site found at 1161
    Illegal BglII site found at 705
    Illegal BamHI site found at 1087
    Illegal XhoI site found at 954
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1161
    Illegal XbaI site found at 1643
    Illegal SpeI site found at 1800
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1161
    Illegal XbaI site found at 1643
    Illegal SpeI site found at 1800
    Illegal AgeI site found at 1725
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None