Composite

Part:BBa_K3748056

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pHHF1-BA-BL-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 1,17257E-05 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 993
    Illegal XbaI site found at 1475
    Illegal SpeI site found at 1632
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 993
    Illegal SpeI site found at 1632
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2256
    Illegal EcoRI site found at 993
    Illegal BglII site found at 705
    Illegal BamHI site found at 919
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 993
    Illegal XbaI site found at 1475
    Illegal SpeI site found at 1632
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 993
    Illegal XbaI site found at 1475
    Illegal SpeI site found at 1632
    Illegal AgeI site found at 1557
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None