Composite

Part:BBa_K3748030

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pREV1-αMF-BA-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct didn't show alpha amylase activity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1173
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1173
    Illegal SpeI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2436
    Illegal EcoRI site found at 1173
    Illegal BglII site found at 705
    Illegal BamHI site found at 1099
    Illegal XhoI site found at 954
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1173
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1173
    Illegal XbaI site found at 1655
    Illegal SpeI site found at 1812
    Illegal AgeI site found at 1737
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None