Composite

Part:BBa_K3748028

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pRNR1-AO-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 9,26803E-05 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 705
    Illegal BglII site found at 2191
    Illegal XhoI site found at 2218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1758
  • 1000
    COMPATIBLE WITH RFC[1000]


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