Composite

Part:BBa_K3748026

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pHHF1-αMF-BS-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 0,001100158 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1590
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1590
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2841
    Illegal EcoRI site found at 1590
    Illegal BglII site found at 705
    Illegal BglII site found at 2062
    Illegal BglII site found at 2232
    Illegal BamHI site found at 2082
    Illegal XhoI site found at 954
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1590
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None