Composite

Part:BBa_K3748025

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pRNR1-BS-tTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 0,00052307 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2691
    Illegal EcoRI site found at 1440
    Illegal BglII site found at 705
    Illegal BglII site found at 1912
    Illegal BglII site found at 2082
    Illegal BamHI site found at 1932
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1440
    Illegal SpeI site found at 732
    Illegal PstI site found at 796
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None