Composite

Part:BBa_K3748021

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pRNR1-αMFΔ-AO-pTDH1

Construct from the library to produce the most active alpha amylase in S.cerevisiae. More information of the full library in "https://2021.igem.org/Team:Groningen/Parts".

The construct showed a mean activity of 3,4293E-05 d[d-nitrophenicol]/dt.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 869
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 705
    Illegal BglII site found at 2305
    Illegal XhoI site found at 2332
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 869
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 869
    Illegal NgoMIV site found at 1872
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None