Part:BBa_K3747600

Nap_operon

Nap_operon from Pseudomonas stutzeri JM300

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 235
  Illegal EcoRI site found at 844
  Illegal EcoRI site found at 2197
  Illegal SpeI site found at 2148
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 235
  Illegal EcoRI site found at 844
  Illegal EcoRI site found at 2197
  Illegal SpeI site found at 2148
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 235
  Illegal EcoRI site found at 844
  Illegal EcoRI site found at 2197
  Illegal BglII site found at 1838
  Illegal BglII site found at 3834
  Illegal XhoI site found at 416
  Illegal XhoI site found at 1519
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 235
  Illegal EcoRI site found at 844
  Illegal EcoRI site found at 2197
  Illegal SpeI site found at 2148
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 235
  Illegal EcoRI site found at 844
  Illegal EcoRI site found at 2197
  Illegal SpeI site found at 2148
  Illegal NgoMIV site found at 172
  Illegal NgoMIV site found at 1193
  Illegal NgoMIV site found at 1963
  Illegal NgoMIV site found at 2810
  Illegal NgoMIV site found at 3112
  Illegal NgoMIV site found at 3602
  Illegal AgeI site found at 655
  Illegal AgeI site found at 2659
  
• 1000
  COMPATIBLE WITH RFC[1000]

Nap is a dissimilatory, periplasmic nitrate reductase which is expressed under aerobic conditions and is required for aerobic denitrification [1]. Nap reduces nitrate (NO₃^-) to nitrite (NO₂^-). Nap is the first enzyme in the denitrification pathway.

references

[1] Ji, B., Yang, K., Zhu, L., Jiang, Y., Wang, H., Zhou, J., & Zhang, H. (2015). Aerobic Denitrification: A Review of Important Advances of the Last 30 Years. Biotechnology and Bioprocess Engineering, 20, 643–651. https://doi.org/10.1007/s12257-015-0009-0