Composite

Part:BBa_K3747204

Designed by: Riemer van der Vliet   Group: iGEM21_Wageningen_UR   (2021-10-21)


Positive control-pL-GFP

GFP is under control of the lux pL promoter, but here luxI is produced under control of the lux pR promoter. Therefore, in high cell densities, the production of GFP should be downregulated, also when no AHL is added to the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 875
    Illegal NheI site found at 898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2321
    Illegal BamHI site found at 520
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None