Composite

Part:BBa_K3747203

Designed by: Riemer van der Vliet   Group: iGEM21_Wageningen_UR   (2021-10-21)


Reporter-pL-RFP

Here GFP is under control of the lux pL promoter which is downregulated by the LuxR-AHL complex. As no AHL is produced by this complex, the promoter is active when there is no AHL in the medium. Where there is AHL in the medium, it should be downregulated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 875
    Illegal NheI site found at 898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 520
    Illegal XhoI site found at 421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None