Composite

Part:BBa_K3747143

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-21)


norCBD Pseudomonas stutzeri JM300

Nor is the third enzyme of the denitrification pathway, converting nitric oxide (NO) into nitrous oxide (N2O).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1947
    Illegal PstI site found at 3072
    Illegal PstI site found at 3481
    Illegal PstI site found at 3946
    Illegal PstI site found at 4276
    Illegal PstI site found at 4297
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
    Illegal PstI site found at 1947
    Illegal PstI site found at 3072
    Illegal PstI site found at 3481
    Illegal PstI site found at 3946
    Illegal PstI site found at 4276
    Illegal PstI site found at 4297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1289
    Illegal BglII site found at 2989
    Illegal BamHI site found at 3750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1947
    Illegal PstI site found at 3072
    Illegal PstI site found at 3481
    Illegal PstI site found at 3946
    Illegal PstI site found at 4276
    Illegal PstI site found at 4297
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1947
    Illegal PstI site found at 3072
    Illegal PstI site found at 3481
    Illegal PstI site found at 3946
    Illegal PstI site found at 4276
    Illegal PstI site found at 4297
    Illegal NgoMIV site found at 3159
    Illegal NgoMIV site found at 3493
    Illegal NgoMIV site found at 3628
    Illegal NgoMIV site found at 4203
    Illegal NgoMIV site found at 4373
    Illegal NgoMIV site found at 5002
    Illegal AgeI site found at 1021
    Illegal AgeI site found at 2598
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None