Composite

Part:BBa_K3747138

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-21)


napEDABC (Periplasmic nitrate reductase) Cupriavidus necator H16

Nap is a dissimilatory, periplasmic nitrate reductase which is expressed under aerobic conditions and is required for aerobic denitrification [1]. Nap is the first enzyme in the denitrification pathway and reduces nitrate (NO3-) to nitrite (NO2-). To establish denitrification in Pseudomonas putida EM42, we expressed this construct and tested its activity to convert NO3- into NO2-.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1557
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1557
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
    Illegal NotI site found at 1044
    Illegal NotI site found at 2884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1557
    Illegal BglII site found at 539
    Illegal BglII site found at 571
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1557
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1557
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
    Illegal NgoMIV site found at 153
    Illegal NgoMIV site found at 288
    Illegal NgoMIV site found at 1125
    Illegal NgoMIV site found at 1261
    Illegal NgoMIV site found at 1856
    Illegal NgoMIV site found at 2613
    Illegal NgoMIV site found at 2684
    Illegal AgeI site found at 1312
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference

[1] Ji, B., Yang, K., Zhu, L., Jiang, Y., Wang, H., Zhou, J., & Zhang, H. (2015). Aerobic Denitrification: A Review of Important Advances of the Last 30 Years. Biotechnology and Bioprocess Engineering, 20, 643–651. https://doi.org/10.1007/s12257-015-0009-0

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