Part:BBa_K3747132

Nitric oxide reductase D (norD) from Pseudomonas stutzeri JM300

This gene is part of the nor operon from P. stutzeri JM300. Nor is the third enzyme of the denitrification pathway, converting nitric oxide (NO) into nitrous oxide (N₂O). NorD has been shown to be essential in Paracoccus denitrificans to get a functional NorCB complex by facilitating non-heme Fe insertion [1]. It is suspected that NorD has a similar function in maturation of NorCB in Pseudomonas aeruginosa and P. stutzeri [1].

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 211
  Illegal PstI site found at 676
  Illegal PstI site found at 1006
  Illegal PstI site found at 1027
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 211
  Illegal PstI site found at 676
  Illegal PstI site found at 1006
  Illegal PstI site found at 1027
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 480
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 211
  Illegal PstI site found at 676
  Illegal PstI site found at 1006
  Illegal PstI site found at 1027
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 211
  Illegal PstI site found at 676
  Illegal PstI site found at 1006
  Illegal PstI site found at 1027
  Illegal NgoMIV site found at 223
  Illegal NgoMIV site found at 358
  Illegal NgoMIV site found at 933
  Illegal NgoMIV site found at 1103
  Illegal NgoMIV site found at 1732
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] M. Kahle, J. ter Beek, J.P. Hosler, P. Ädelroth, The insertion of the non-heme Fe B cofactor into nitric oxide reductase from P . denitrificans depends on NorQ and NorD accessory proteins, BBA - Bioenerg. 1859 (2018) 1051–1058. https://doi.org/10.1016/j.bbabio.2018.05.020.