Coding

Part:BBa_K3747131

Designed by: Anemoon Drinkwaard   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitric oxide reductase B (norB) from Pseudomonas stutzeri JM300

This gene is part of the nor operon from P. stutzeri JM300. Nor is the third enzyme of the denitrification pathway, converting nitric oxide (NO) into nitrous oxide (N2O). NorB is a cytochrome oxidizing enzyme, which forms a complex with NorC [1]. Together they form the active subunit of Nor.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 115
    Illegal PstI site found at 1240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 115
    Illegal PstI site found at 1240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1157
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 115
    Illegal PstI site found at 1240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 115
    Illegal PstI site found at 1240
    Illegal NgoMIV site found at 1327
    Illegal AgeI site found at 766
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] L. Philippot, Denitrifying genes in bacterial and Archeal genomes, Biochim. Biophys. Acta (BBA)-Gene Struct. Expr. 1577 (2002) 355–376

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