Coding

Part:BBa_K3747129

Designed by: Anemoon Drinkwaard   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrite reductase V (nirV) from Pseudomonas stutzeri JM300

This gene is part of the nir operon from P. stutzeri JM300. Nir is the second enzyme of the denitrification pathway, converting nitrite (NO2-) into nitric oxide (NO). NirK nitrite reductases are copper-dependent [1]. It has long been thought the presence of NirS (a heme-dependent Nir) and NirK was mutually exclusive, however, this is not true as for example Pseudomonas stutzeri possesses both [2]. In contrast to NirS, NirK does not need any other genes for its maturation or for it to be functionally active. Nevertheless, in some organisms, e.g. P. stutzeri JM300, nirK gene is accompanied by nirV gene. The exact function of nirV is not elucidated yet as of today [3,4], but it is recognized as a known accessory gene of NirK [20].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 487
    Illegal NgoMIV site found at 541
    Illegal NgoMIV site found at 907
    Illegal NgoMIV site found at 991
    Illegal AgeI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] L. Wittorf, C.M. Jones, G. Bonilla-Rosso, S. Hallin, Research in Microbiology Expression of nirK and nirS genes in two strains of Pseudomonas stutzeri harbouring both types of NO-forming nitrite reductases, Res. Microbiol. 169 (2018) 343–347. https://doi.org/10.1016/j.resmic.2018.04.010.

[2] D. Helen, H. Kim, B. Tytgat, W. Anne, Highly diverse nirK genes comprise two major clades that harbour ammonium- producing denitrifiers, BMC Genomics. 17 (2016) 1–13. https://doi.org/10.1186/s12864-016-2465-0.

[3] L. Philippot, Denitrifying genes in bacterial and Archeal genomes, Biochim. Biophys. Acta (BBA)-Gene Struct. Expr. 1577 (2002) 355–376.

[4] R. Jain, J.P. Shapleigh, Characterization of nirV and a gene encoding a novel pseudoazurin in Rhodobacter sphaeroides 2 .4 .3, Microbiology. 147 (2001) 2505–2515.

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