Coding

Part:BBa_K3747125

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Blue (type-1) copper protein from Pseudomonas stutzeri JM300 (PSJM300_10725)

Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N2O) into dinitrogen gas (N2). Blue (type 1) copper domain-containing proteins are possible electron donors for NosZ, the catalytic subunit of Nos [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 241
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 241
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 241
    Illegal NgoMIV site found at 67
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] S. Dell’Acqua, I. Moura, J. J. G. Moura, and S. R. Pauleta, “The electron transfer complex between nitrous oxide reductase and its electron donors,” JBIC J. Biol. Inorg. Chem. 2011 168, vol. 16, no. 8, pp. 1241–1254, Jul. 2011, doi: 10.1007/S00775-011-0812-9.

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