Part:BBa_K3747124

ApbE from Pseudomonas stutzeri JM300

Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N₂O) into dinitrogen gas (N₂). The nos gene cluster of P. stutzeri contains nosR, which encodes a membrane protein serving as electron donor for N₂O reduction [1]. This protein requires covalent flavinylation, which is hypothesized to be performed by ApbE in P. stutzeri [2],[3].

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 964
  Illegal PstI site found at 163
  Illegal PstI site found at 463
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 964
  Illegal PstI site found at 163
  Illegal PstI site found at 463
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 964
  Illegal BamHI site found at 786
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 964
  Illegal PstI site found at 163
  Illegal PstI site found at 463
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 964
  Illegal PstI site found at 163
  Illegal PstI site found at 463
  Illegal AgeI site found at 796
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1]P, W., & WG, Z. (2005). Functional domains of NosR, a novel transmembrane iron-sulfur flavoprotein necessary for nitrous oxide respiration. Journal of Bacteriology, 187(6), 1992–2001. https://doi.org/10.1128/JB.187.6.1992-2001.2005

[2]L, Z., C, T., SLA, A., & O, E. (2017). The flavinyl transferase ApbE of Pseudomonas stutzeri matures the NosR protein required for nitrous oxide reduction. Biochimica et Biophysica Acta. Bioenergetics, 1858(2), 95–102. https://doi.org/10.1016/J.BBABIO.2016.11.008

[3]Wunsch, P., Herb, M., Wieland, H., Schiek, U. M., & Zumft, W. G. (2003). Requirements for CuA and Cu-S center assembly of nitrous oxide reductase deduced from complete periplasmic enzyme maturation in the nondenitrifier Pseudomonas putida. Journal of Bacteriology, 185(3), 887–896. https://doi.org/10.1128/JB.185.3.887-896.2003