Part:BBa_K3747123

Twin-arginine translocation A (tatA) from Pseudomonas stutzeri JM300

This gene is part of the nos operon from P. stutzeri JM300. Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N₂O) into dinitrogen gas (N₂). TatA supports Tat-dependent export of apo-NosZ into the periplasm [1],[2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] Wunsch, P., Herb, M., Wieland, H., Schiek, U. M., & Zumft, W. G. (2003). Requirements for CuA and Cu-S center assembly of nitrous oxide reductase deduced from complete periplasmic enzyme maturation in the nondenitrifier Pseudomonas putida. Journal of Bacteriology, 185(3), 887–896. https://doi.org/10.1128/JB.185.3.887-896.2003

[2] Zhang, L., Wüst, A., Prasser, B., Müller, C., & Einsle, O. (2019). Functional assembly of nitrous oxide reductase provides insights into copper site maturation. Proceedings of the National Academy of Sciences of the United States of America, 116(26), 12822–12827. https://doi.org/10.1073/pnas.1903819116