Coding

Part:BBa_K3747122

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrous oxide reductase L (nosL) from Pseudomonas stutzeri JM300

This gene is part of the nos operon from P. stutzeri JM300. Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N2O) into dinitrogen gas (N2). NosL is a copper chaperone, which binds copper and subsequently delivers it to apo-NosZ [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 247
    Illegal PstI site found at 493
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 247
    Illegal PstI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 247
    Illegal PstI site found at 493
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 247
    Illegal PstI site found at 493
    Illegal NgoMIV site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] LM, T., MA, M., DM, D., & V, C. (2006). Structural studies of Apo NosL, an accessory protein of the nitrous oxide reductase system: insights from structural homology with MerB, a mercury resistance protein. Biochemistry, 45(40), 12240–12252. https://doi.org/10.1021/bi061089+

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