Coding

Part:BBa_K3747121

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrous oxide reductase Y (nosY) from Pseudomonas stutzeri JM300

This gene is part of the nos operon from P. stutzeri JM300. Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N2O) into dinitrogen gas (N2). NosY together with NosF form an ABC transporter, which is presumably required for the shuttling of sulfur species to the periplasm for assembly of NosZ [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 118
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 523
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] Honisch, U., & Zumft, W. G. (2003). Operon Structure and Regulation of the nos Gene Region of Pseudomonas stutzeri, Encoding an ABC-Type ATPase for Maturation of Nitrous Oxide Reductase. Journal of Bacteriology, 185(6), 1895. https://doi.org/10.1128/JB.185.6.1895-1902.2003

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