Coding

Part:BBa_K3747120

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrous oxide reductase F (nosF) from Pseudomonas stutzeri JM300

This gene is part of the nos operon from P. stutzeri JM300. Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N2O) into dinitrogen gas (N2). NosF together with NosY form an ABC transporter, which is presumably required for the shuttling of sulfur species to the periplasm for assembly of NosZ [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 157
    Illegal PstI site found at 616
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 157
    Illegal PstI site found at 616
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 337
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 157
    Illegal PstI site found at 616
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 157
    Illegal PstI site found at 616
    Illegal NgoMIV site found at 118
    Illegal NgoMIV site found at 655
    Illegal NgoMIV site found at 892
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] Honisch, U., & Zumft, W. G. (2003). Operon Structure and Regulation of the nos Gene Region of Pseudomonas stutzeri, Encoding an ABC-Type ATPase for Maturation of Nitrous Oxide Reductase. Journal of Bacteriology, 185(6), 1895. https://doi.org/10.1128/JB.185.6.1895-1902.2003

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