Coding

Part:BBa_K3747118

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrous oxide reductase Z (nosZ) from Pseudomonas stutzeri JM300

This gene is part of the nos operon from P. stutzeri JM300. Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N2O) into dinitrogen gas (N2). NosZ encodes the catalytic subunit, which converts N2O into N2O [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1523
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 544
    Illegal PstI site found at 628
    Illegal PstI site found at 1851
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 1078
    Illegal NgoMIV site found at 1656
    Illegal AgeI site found at 88
    Illegal AgeI site found at 900
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] Wüst, A., Schneider, L., Pomowski, A., Zumft, W. G., Kroneck, P. M. H., & Einsle, O. (2012). Nature’s way of handling a greenhouse gas: the copper-sulfur cluster of purple nitrous oxide reductase. Biological Chemistry, 393(10), 1067–1077. https://doi.org/10.1515/HSZ-2012-0177

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Parameters
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