Coding

Part:BBa_K3747117

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Nitrous oxide reductase R (nosR) from Pseudomonas stutzeri JM300

This gene is part of the nos operon from P. stutzeri JM300. Nos is the last enzyme of the denitrification pathway, converting nitrous oxide (N2O) into dinitrogen gas (N2). NosR encodes a membrane protein serving as electron donor for N2O reduction [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1030
    Illegal NheI site found at 1540
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1030
    Illegal BglII site found at 1970
    Illegal XhoI site found at 334
    Illegal XhoI site found at 1099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1030
    Illegal PstI site found at 1330
    Illegal PstI site found at 1600
    Illegal NgoMIV site found at 258
    Illegal NgoMIV site found at 591
    Illegal AgeI site found at 1909
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] P, W., & WG, Z. (2005). Functional domains of NosR, a novel transmembrane iron-sulfur flavoprotein necessary for nitrous oxide respiration. Journal of Bacteriology, 187(6), 1992–2001. https://doi.org/10.1128/JB.187.6.1992-2001.2005

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