Coding

Part:BBa_K3747112

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Periplasmic nitrate reductase A (napA) from Paracoccus denitrificans DSM 413

This gene is part of the nap operon from P. denitrificans DSM 413. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO3-) into nitrite (NO2-). NapA is the catalytic subunit [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 254
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Coelho, C., & Romão, M. J. (2015). Structural and mechanistic insights on nitrate reductases. Protein Science, 24(12), 1901–1911. https://doi.org/10.1002/PRO.2801

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