Coding

Part:BBa_K3747109

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Periplasmic nitrate reductase C (napC) from Cupriavidus necator H16

This gene is part of the nap operon from C. necator H16. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO3-) into nitrite (NO2-). NapC is a tetra-heme transmembrane protein and is also considered an essential component of the electron transfer chain to NapA in organisms in which the gene is found [1].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal NgoMIV site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1] LC, P., & JA, C. (1999). Essential roles for the products of the napABCD genes, but not napFGH, in periplasmic nitrate reduction by Escherichia coli K-12. The Biochemical Journal, 344 Pt 1(Pt 1), 69–76. https://doi.org/10.1042/0264-6021:3440069

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Categories
Parameters
None