Coding

Part:BBa_K3747107

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Periplasmic nitrate reductase A (napA) from Cupriavidus necator H16

This gene is part of the nap operon from C. necator H16. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO3-) into nitrite (NO2-). NapA is the catalytic subunit [1].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 991
    Illegal PstI site found at 931
    Illegal PstI site found at 1396
    Illegal PstI site found at 2449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 991
    Illegal PstI site found at 931
    Illegal PstI site found at 1396
    Illegal PstI site found at 2449
    Illegal NotI site found at 478
    Illegal NotI site found at 2318
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 991
    Illegal BglII site found at 5
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 991
    Illegal PstI site found at 931
    Illegal PstI site found at 1396
    Illegal PstI site found at 2449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 991
    Illegal PstI site found at 931
    Illegal PstI site found at 1396
    Illegal PstI site found at 2449
    Illegal NgoMIV site found at 559
    Illegal NgoMIV site found at 695
    Illegal NgoMIV site found at 1290
    Illegal NgoMIV site found at 2047
    Illegal NgoMIV site found at 2118
    Illegal AgeI site found at 746
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Coelho, C., & Romão, M. J. (2015). Structural and mechanistic insights on nitrate reductases. Protein Science, 24(12), 1901–1911. https://doi.org/10.1002/PRO.2801

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