Coding

Part:BBa_K3747102

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-20)


Periplasmic nitrate reductase A (napA) from Pseudomonas stutzeri JM300

This gene is part of the nap operon from P. stutzeri JM300. Nap is the first enzyme of the denitrification pathway, converting nitrate (NO3-) into nitrite (NO2-). NapA is the catalytic subunit [1].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 1699
    Illegal SpeI site found at 1650
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 1699
    Illegal SpeI site found at 1650
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 1699
    Illegal BglII site found at 1340
    Illegal XhoI site found at 1021
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 1699
    Illegal SpeI site found at 1650
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 346
    Illegal EcoRI site found at 1699
    Illegal SpeI site found at 1650
    Illegal NgoMIV site found at 695
    Illegal NgoMIV site found at 1465
    Illegal NgoMIV site found at 2312
    Illegal AgeI site found at 157
    Illegal AgeI site found at 2161
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Coelho, C., & Romão, M. J. (2015). Structural and mechanistic insights on nitrate reductases. Protein Science, 24(12), 1901–1911. https://doi.org/10.1002/PRO.2801

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