Part:BBa_K3746004

NSP4 Signal Peptide

This sequence was developed by Han, S.K., et al. (2017). [1]

A new signal sequence for E. coli protein secretion. From the literature, the secretion efficiency of NSP4 is about 3.5 fold higher than commonly used signal peptides such as DsbAss.

Meanwhile, >80% of the secreted protein can be purified from culture medium instead of trapping in periplasmic space. This feature makes NSP4 an ideal signal peptide for our project to secrete AFB-1 degrading enzymes.

Reference

[1] Han, S. J., Machhi, S., Berge, M., Xi, G., Linke, T., & Schoner, R. (2017). Novel signal peptides improve the secretion of recombinant Staphylococcus aureus alpha toxinH35L in Escherichia coli. AMB Express, 7(1). https://doi.org/10.1186/s13568-017-0394-1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 48
  
• 1000
  COMPATIBLE WITH RFC[1000]