Part:BBa_K3743010
DegraderToeHold
Part Description
Toehold switches are synthetic RNAs that mimic messenger RNAs whose job it is to shuttle information from the DNA to the protein-synthesizing machinery. They contain a recognition sequence (toehold) for a specific stimulus in form of a specific “input” RNA, and a recognition sequence that the protein-synthesizing machinery (ribosome) needs to bind to initiate the translation of a fused protein-coding sequence into its encoded protein product. In the absence of the “input” RNA, the toehold switch is kept in its OFF state by forming a hairpin structure that uses part of the “input” recognition sequence and the ribosome recognition sequence, which is kept inaccessible.
Usage
This is a downstream switch which binds to miRNA that indicates tumor suppression. It is modified by replacing the liker with malate 1 triplex and adding 30X degradation motifs downstream. This enables degradation of the transcripts to decrease the yield of the vaccine after suppressing the tumor.
Structural Characterization
In order to model the structural stability of DegraderToeHold switch, we used RNAfold tool that predicts secondary structures of single stranded RNA.
The free energy of the thermodynamic ensemble is -124.84 kcal/mol.
The frequency of the MFE structure in the ensemble is 0.09 %.
The ensemble diversity is 60.44 .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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