Part:BBa_K3743008
ToeHold switch to be fused with x30 degradation motif
Part description
Toehold switches are synthetic RNAs that mimic messenger RNAs whose job it is to shuttle information from the DNA to the protein-synthesizing machinery. They contain a recognition sequence (toehold) for a specific stimulus in form of a specific “input” RNA, and a recognition sequence that the protein-synthesizing machinery (ribosome) needs to bind to initiate the translation of a fused protein-coding sequence into its encoded protein product. In the absence of the “input” RNA, the toehold switch is kept in its OFF state by forming a hairpin structure that uses part of the “input” recognition sequence and the ribosome recognition sequence, which is kept inaccessible.
That would be combined with Protein degradation motifs to modify it's function
Usage
It's used to create a system-sensitive circuit using ToeHold switches both upstream and downstream of the vaccination. To remove the inhibitory impact of the Riboswitch and create more copies of transcripts to battle cancer cells, the ToeholdOnmotif switch upstream of vaccination binds to miRNA suggesting enhanced tumour growth. Another downstream ToeholdDegmotifs binds to suppressor miRNA, causing transcripts to be degraded and vaccine copies to be reduced.
Structural Characterization
In order to model the structural stability of DegraderToeHold switch, we used RNAfold tool that predicts secondary structures of single stranded RNA.
The free energy of the thermodynamic ensemble is -124.84 kcal/mol.
The frequency of the MFE structure in the ensemble is 0.09 %.
The ensemble diversity is 60.44 .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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