Regulatory

Part:BBa_K374017

Designed by: Annemi Jollmann, Anja Sander, Maya Friis Kjaergaard, Lisa Blanc Iversen and Grzegorz Słodkowicz   Group: iGEM10_DTU-Denmark   (2010-10-27)


Gifsy2 anti-repressor which anti-represses the Gifsy2 repressor gtgR

Gifsy2 is a temperate phage present many of the Salmonella enterica serovar Typhimurium strains. This part contains the anti-repressor from Gifsy2 called antT which inactivates the repressor from BioBrick K_374009 resulting in transcription from the pR Gifsy2 promoter. AntT works by direct non-covalent binding to gtgR and prevents its DNA binding activity.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 21
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None