Part:BBa_K3738016
MS2 Bacteriophage Capsid Protein with C-Terminal Histidine Tag (Composite Part)
The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as one copy of maturation protein A (BBa_K3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, MS2 capsid protein coding region with a C-terminal histidine tag (BBa_K3738006), and double terminator BBa_B0015. The MS2 capsid protein coding region in this part includes a histidine tag on the C-terminus for to allow for peptide purification.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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