Insulin is a kind of protein that can make blood glucose level down.
We have proved that Insulin can be processed and secreted outside the cell in 293T cells.
Fig.1 The model diagram of 9XUAS-Insulin
In the experiment, we used an ELIAS kit specially designed to detect human mature insulin to detect cell eliquidation.
Insulin can be processed and secreted outside the cell in 293T cells.
Fig.2 Standard curve of absorbance and insulin concentration
Fig.3 Insulin concentration in supernatant after blue light irradiation and dark treatment respectively
Fig.4 Insulin concentration in supernatant (excluding insulin in medium) after blue light irradiation and dark treatment respectively
Insulin is not suitable for long time preservation under the best culture temperature of cells (37℃). In the mean time, cells would also consume some of the expressed insulin. So the best tst time for Insulin is approximately 24 hours after transfection, it is relatively shorter than the test interval of LUC.
Mingqi Xie, Haifeng Ye, Hui Wang.β-cell-mimetic designer cells provide closed-loop glycemic control[J].Science.2016 Dec 9;354(6317):1296-1301.
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC