Part:BBa_K3734014
LUC
Luciferin(LUC) is a protein that can activate fluorescein.We use it as reporter gene.
LUC
LUC is a reported gene in the luminescence system of fluorease. It comes from fireflies and characterizes the amount of gene expression in the target by detecting
the luminescence value of its excited fluorescent hormone at a wavelength of 560nm. Let's take LUC after CHREBP promoter as an example to prove that LUC can
complete the task of reporting genes well.
1.Pattern diagram
Fig.1 The model diagram of CHREBP-LUC
2.Experiments
2.1 Method
We used LUC as report genes to reflect the level of expression through detecting luminescence value at 560nm wavelength and the error REN luminescence caused by the number of eliminated cells.
2.2 Results
The experiments proved that LUC can complete the mission of being a report gene well.
Fig.2 The expression level of inducible promoter CHREBP was respectively analyzed at 48h in 25mM, 5.6mm and 0mM glucose culture
3.Caution
Try to convert REN at the same time to adjust the errors caused by other factors such as different cell numbers and cell states caused by different culture conditions.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 64
Illegal NgoMIV site found at 1408
Illegal NgoMIV site found at 1429
Illegal AgeI site found at 1132 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1314
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