ELK is a kind of protein that can activate downstream gene expression after being regulated by phosphorylation.
ELK is generally dormant, when INSR accepts insulin to activate the MAPK pathway, Elk is phosphated into an active state, which can activate the expression of the gene downstream TRE.
Fig.1 The model diagram TetR-ELK
We used Western Blot to detect phosphorylation of ERK1/ERK2 in the phosphorylation pathway with ERK antibodies.
Fig.2 ERK phosphorylation changes with different insulin treatment
Fig.3 ERK phosphorylation changes with different insulin treatment
Despite the length and the complication of phosphorylation pathway, the phosphorylation pathway of protein is a very short process, it is usually completed within minutes even seconds. The time of cracking the cells and collecting protein must be controlled precisely when detecting.
Haifeng Ye, Mingqi Xie, Shuai Xue.Self-adjusting synthetic gene circuit for correcting insulin resistance[J].Nat Biomed Eng.2017 Jan;1(1):0005.
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