GIP is a promoter that can be induced by glucose. We first use it in 293T cell.
GIP promoter is anticipated in current literature that it can only be used in several certain types of cells like intestine cells, but we proved that it can function will in 293T cells which are usually used in labs.
Fig.1 The model diagram of CHREBP-LUC
We use LUC as report gene, test its real-time luminescence value at 560nm wavelength with specific devices and detect the expression level under different glucose concentration.
GIP promoter is induced by glucose and the expression level rises along with the raise of glucose concentration.
Fig.2 Changes of LUC expression over time after blue light irradiation with different glucose concentrations
Use medium without glucose when paving in case of the effect caused by glucose residue
Anthony T. Cheung,Bama Dayanandan,Jamie T. LewisGlucose-Dependent Insulin Release from Genetically Engineered K Cells[J].Science.2000,290:1959-1962
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 899
- 1000Illegal BsaI.rc site found at 216