Part:BBa_K3731007
vgb+mazE
This composite part is the combination of vgb and mazE gene, which are helpful for the growth of bacteria.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
iGEM2021_Nanjing-China Experiment
Group: Nanjing-China 2021
Author: Hao Yin
Besides, the structure of mazE antitoxin is drawn by PyMol as is shown below.
The mazE protein is such a useful antitoxin that it can help bacteria grow longer and survive harsher environment, thus producing more polyP. Thus, we insert mazE gene into E. coli genome to increase the productivity.
Bacterial hemoglobin (VtHb) is produced by the gram-negative bacterium, Vitreoscilla , in large quantity in response to hypoxic environmental conditions. The vgb gene coding for VtHb has been cloned in E. coli where it is expressed strongly by its natural promoter. The expression of the vgb gene in Vitreoscilla is transcriptionally regulated by oxygen. Vitreoscillu hemoglobin (VHb) is expressed at elevated levels in Vitreoscillu under oxygen-limited cultivation conditions.
Adding vgb and mazE into the gene helps the growth of bacteria, thus improving the productivity of polyP.
Reference: Zorzini, Buts et al. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding, Nucleic Acids Research, Volume 43, Issue 2, 30 January 2015, Pages 1241–1256
Kallio, P. T.; Kim, D.; Tsai, P. S.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions. The FEBS Journal 1994, 219 (1-2), 201-208.
Kanak L. Dikshit, Rajendra P. Dikshit, Dale A. Webster, Study of Vitreoscilla globin(vgb) gene expression and promoter activity in E. Coli through transcriptional fusion , Nucleic Acids Research, Volume 18, Issue 14, 25 July 1990, Pages 4149–4155.
| None |