Primer

Part:BBa_K3730034

Designed by: Guanrui Huang   Group: iGEM21_ZJU-China   (2021-09-14)


pcDNA3.1-N-eGFP-R

Use with BBa_K3730033. This couple of primer can be used to verify the inserting sequence in pcDNA3.1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 20
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None