Composite

Part:BBa_K3729004

Designed by: Austin Wang, Yi-Chen Lin, Sinswien Lee   Group: iGEM21_KCIS_NewTaipei   (2021-10-17)


Strong promoter + strong rbs + BCD 2 + double terminator

The composite part consists of a strong promoter (BBa_J23100), a strong ribosome binding site(BBa_K880005), our BCD2 gene (BBa_K3729001), and a double terminator (BBa_B0015). This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl- CoA dehydrogenase. Our composite part is synthesized by IDT.

<img src="T--KCIS_New_Taipei--BCD2_complex.png" />

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 156
    Illegal SpeI site found at 273
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 156
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 50
    Illegal NheI site found at 73
    Illegal SpeI site found at 273
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 156
    Illegal BamHI site found at 727
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 156
    Illegal SpeI site found at 273
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 156
    Illegal SpeI site found at 273
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None