Part:BBa_K3729001

BCD 2: (Butyryl- CoA Dehydrogenase)

Butyryl- CoA dehydrogenase catalyzes the rate-limiting step (Crotonyl-CoA to butyryl-CoA) of the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (BCD) encoded for butyryl- CoA dehydrogenase from the genome of Clostridium acetobutylicum.

Construct design:We optimized the DNA sequence for expression in E. coli and flanked the open reading frame with an upstream strong promoter, a strong ribosome binding site (RBS), and a double terminator. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of butyryl-CoA dehydrogenase. Our composite part is synthesized by IDT.

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