Intermediate

Part:BBa_K3725030

Designed by: Andrew Bae, Pranav Kanthala, Monica Cho, Janet Standeven, Megan McSweeney   Group: iGEM21_Lambert_GA   (2021-09-28)

Phytophthora Trigger Sequence
Overview
This part is a fragment of the X24 gene of the Phytophthora cryptogea species. This fragment is intended to be used in order to interact with the Phytophthora Toehold w/ GFP Reporter (Part BBa_K3725010) to unravel the hairpin loop in the switch, and allow for expression of the GFP reporter protein downstream. As a result, this part is used to test the functionality of the toehold part in sensing the X24 gene and being a working biosensor for the P. cryptogea pathogen.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None