Coding

Part:BBa_K371031

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-10-27)


MPF(meta-prefix)+[pduV98+10*GS+GFP] fusion protein+MSF(meta-suffix)

This parts is a fusion protein between the N terminus 98 amino acid of pdu V protein and RFP protein(BBa_E1010). At two side is special DNA sequence that make this parts compatible to BBF RFC 53. The MPF and MSF is the abbreviation of meta-prefix and meta-suffix seperately. Because the N terminus is said to target pduV outside the pdu BMC, this part can be used to direct pduV98-GFP chimera onto BMC surface.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 872
    Illegal SapI site found at 3


[edit]
Categories
Parameters
n/aMPF(meta-prefix)+[pduV98+10*GS+GFP] fusion protein+MSF(meta-suffix)