Composite

Part:BBa_K3710006

Designed by: Sandra Lizeth Martinez Diaz   Group: iGEM21_Manchester   (2021-10-01)


LPP-OmpA with an integrated Cellulose-binding Domain (Cex) from Cellulomonas fimi

To bind our chassis to a cellulose-based hydrogel coating for urinary catheters, we designed the composite part LPP-OmpA-CBD(cex) (BBa_K863101) comprising the Cellulose Binding Domain (CBD) on the exoglucanase/xylanase (Cex) from Cellulomonas fimi (BBa_K863101) fused into an LPP-OmpA-N hybrid (BBa_K1694002).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 454
    Illegal NgoMIV site found at 514
    Illegal AgeI site found at 847
  • 1000
    COMPATIBLE WITH RFC[1000]


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