Regulatory

Part:BBa_K3710003

Designed by: Luke Roche, Sandra Martinez Diaz, Erik Hanko and Katherine Baker   Group: iGEM21_Manchester   (2021-09-29)


Urea-inducible promoter PureD

This part harbours the inducible promoter PureD, which is activated by the UreR transcriptional regulator in the presence of urea. It is one of several promoters activated by the UreR transcriptional activator which share a consensus sequence of TA/GT/CA/TT/GC/TTA/TT/AATTG [1].

PureD is a bidirectional promoter driving transcription of the ureR and ureD genes of P. mirabilis. UreR binding sites are located from -110 to -96 and from -44 to -29 bp upstream of ureD tranlational start site.

References:

[1] Thomas, V. J., & Collins, C. M. (1999). Identification of UreR binding sites in the Enterobacteriaceae plasmid‐encoded and Proteus mirabilis urease gene operons. Molecular Microbiology, 31(5), 1417-1428.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation
Parameters
None