Composite

Part:BBa_K3700009

Designed by: Juliette Moreau   Group: iGEM20_Montpellier   (2020-10-14)


azurin+sfGFP with pTAC promoter and TAT secretion signal +features

sfGFP linked to the Azurin gene with a secretion signal and an inducible promoter surrounded by spacers in order to create universal primers. Verification of the expression and the secretion of the azurin thanks to the sfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1497
    Illegal BamHI site found at 1647
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 741


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Categories
Parameters
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